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KMID : 1025520080500060849
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Journal of Animal Science and Technology
2008 Volume.50 No. 6 p.849 ~ p.856
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cDNA Microarray Analysis of Gene Expression in Pig Spleen Lymphocytes in Response to Extract of Raspberry
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Chung Chung-Soo
Choi Young-Suk
Lim Hee-Kyong
O Baatartsogt.
Mandal Prabhat-Kumar
Choi Kang-Duk
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Abstract
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The present study was undertaken to investigate specific immune response of Rubus coreanus Miquel (raspberry) in pig spleen lymphocytes and gene expression induced by the extracts of raspberry using gene chip technology. The 70% ethyl alcohol extracts of raspberry were treated to pig spleen lymphocytes. The extracts of raspberry stimulated the proliferation of splenocytes and increased the population of CD3 & CD4 T-cells and B-cells in pig spleen lymphocytes. The extracts of raspberry improved immune response by increasing the viability of splenocytes. In microarray study we found eight genes were significantly up- regulated by the extracts of raspberry in pig splenocytes, including genes known to be involved in cell structure and immune response, particularly microtubule-associated protein 4, cytoplasmic dynein heavy chain, tumor necrosis factor alpha, lymphotoxin-beta receptor precursor. However, ten genes were down- regulated by the extracts of raspberry treatment.
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KEYWORD
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Raspberry, Pig, Lymphocyte, Microarray, Gene expression
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